Processes yielding >500 mg plasmid DNA/L have previously been reported by Merck, Boehringer Ingelheim, and Nature Technology (NTC). Common features among these high-yield processes include the use of ...

Figure 1: Concept of the systems metabolic engineering framework. Figure 3: General scheme of systems metabolic engineering and its case study on the overproduction of 1,4-butanediol using E. coli 11.

Saccharomyces cerevisiae has long been grown in fed-batch cultivation by the brewing and baking industry, where it was selected principally for its vigorous growth. It has also been used in the ...

The utilization of online process analytical technology (PAT) has become a high-profile endeavor in the biotechnology industry in recent years. Since the early 1980s, many fermentation scientists have ...