Microbiology Laboratory offers a rapid detection and identification of microorganisms using Enzyme Substrate Test (EST). It is one of the approved methods by the Department of Health (DOH) and Department of Environment and Natural Resources (DENR) on the Analysis of Drinking Water and Wastewater. It detects the presence of microorganisms such as Total Coliforms and Escherichia coli. Enzyme Substrate Test is a testing wherein the hydrolyzable chromogenic and fluorogenic substrate is added to the